Coding

Part:BBa_K1685005:Design

Designed by: ITB_Indonesia 2015   Group: iGEM15_ITB_INDONESIA   (2015-09-16)


aeBlue


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 281
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 390
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The part has been codon-optimised for expression in E. coli using http://sg.idtdna.com/CodonOpt


Source

The aeBlue sequence from Uppsala team 2012 (BBa_K864401) was chemically synthesised.

References